effects of various activators on bovine embryonic development following intracytoplasmic sperm injection
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abstract
the objective of this study was to compare the effectiveness of various combination activators of bovine oocyte following intracytoplasmic sperm injection (icsi) in terms of cleavage and embryo development. bovine icsi oocytes were treated with a variation of treatments of combined or single activators as follows; 30 v for 15 x 2 μsec of electrical pulse +10 μg/ml cycloheximide (chx),7% ethanol + chx, 5 μm calcium ionophore a23187 (ca-i)+ chx, ca-i + 1.9 mm 6-dimethylaminopurine (6-dmap), chx alone, ca-i alone. the highest cleavage (73.5%-79.7%), and blastocyst rates (28.0%-35.3%) were obtained in ca-i + 6-dmap, ethanol + chx and electrical stimuli + chx groups. the treatment with chx only or ca-i only gave a significantly lower (p
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Journal title:
iranian journal of applied animal sciencePublisher: islamic azad university - rasht branch
ISSN 2251-628X
volume 2
issue 2 2015
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